HPLC and GC - what is the difference - animation HD Video
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The difference between HPLC (High Pressure Liquid Chromatography) and GC (Gas Chromatography) can be described in four main differences.
Learn the basic concept of High Pressure Liquid Chromatography
01. Introduction
https://youtu.be/IUwRWn9pEdg
02. The Mobile Phase
https://youtu.be/pmHtGDdagJU
03. The Stationary Phase
https://youtu.be/MYSBOxbnuAw
04. Normal Phase HPLC vs Reverse Phase HPLC
https://youtu.be/MLoitPJQH3g
05. HPLC Isocratic vs Gradient analysis
http://youtu.be/tAcfJPveWwM
06. HPLC - UV-VIS detection of analytes
https://youtu.be/sfxEj_MxBcs
07. HPLC - How to read a chromatogram?
https://youtu.be/qXmSb6Xwr5k
08. What is the difference between HPLC and GC?
https://www.youtube.com/watch?v=FlTf2BRtR2s
1. Mobile phase
HPLC uses liquid as carrier and GC uses gas as carrier.
2. Column
HPLC uses a column with a length between 5 to 25 cm. In GC analysis columns from 10 to 30 meters are used.
3. Separation
In HPLC separation takes places based on the polarity of the compounds. In GC the compounds are often forced to separate with temperature.
4. Compounds
Generally, non-volatile analytes cannot be directly analyzed using GC. Sometimes derivatization will be helpful to turn non-volatile compounds into volatile ones to be analyze using GC, but it does not always work.
HPLC, using liquid as mobile phase, can analyze compounds which can be dissolved in liquid. Volatility is not that important here. Immediately, we see HPLC can be used on much more analytes than GC, because there are a lot of non-volatile compounds can be dissolved (at least a little bit) in water, methanol, acetonitrile, acetone, or hexane, etc. Therefore, currently, HPLC is used more often than GC.
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